![]() The MILLIPLEX® MAP Akt/mTOR Phosphoprotein 11-plex Magnetic Bead Kit (#48-611MAG) was used in conjunction with MILLIPLEX® MAP Phospho ERK/MAPK 1/2 (Thr185/Tyr187) Magnetic Bead MAPmate™ and MILLIPLEX® MAP Total β-Tubulin Magnetic Bead MAPmate to simultaneously detect 12 phosphoproteins with β-tubulin serving as a loading control. To maximize efficiency and data output from our limited sample, a multiplex cell signaling assay was run on the Luminex® platform as an alternative to Western blotting. Hodge, in International Review of Neurobiology, 2019 2.9 Multiplex assay for the Akt/mTOR signaling pathway ![]()
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